A KASP assay was developed to detect the G143A mutation in Stemphylium vesicarium, linked to QoI fungicide resistance. The assay was applied to 70 single-spore isolates from onion fields in Ontario from 1995 to 2023, and to DNA from air samples collected in 2021-2024. The G143A mutation was found in 67 of 70 historical isolates and in all isolates from 2023-2024 Burkard collections.
Use Cases
- Tracking the historical prevalence of a fungicide resistance mutation based on isolate collection data spanning nearly three decades.
- Validating molecular detection assays for pathogen monitoring based on the described KASP assay for the G143A SNP.
- Correlating airborne spore data with fungicide resistance profiles based on DNA extracted from Burkard sampler collections.
Strengths
- Data covers a long temporal range, with isolates collected from 1995 to 2023.
- The assay validation includes results from 70 historical isolates and from environmental air sampling.
Limitations
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count is unknown, which may limit suitability assessment.
Provenance
- Source
- Borealis Harvested Dataverse
- Collection Method
- Kompetitive Allele Specific PCR (KASP) assay applied to lab-isolated fungal samples and environmental DNA.
- Time Range
- 1995 to 2024
- Freshness
- Last updated 2026-06-13 04:11:04; freshness should be verified.
- Geography
- Holland Marsh and Bradford, Ontario, Canada