Supplementary Data for Central Fractalkine Signaling Study in Female Rats

Supplementary images and probe sequences for a neuroscience study investigating fractalkine signaling. The dataset includes in situ hybridization images from the brains of ovariectomized, estradiol-treated rats and a table of probe sequences. It was authored by Yuki Otsuka and last updated on May 31, 2026.

Use Cases

• Validate probe specificity for in situ hybridization experiments based on the provided sense probe control images.
• Reference probe sequences for replicating HCR experiments targeting Cx3cl1 and Cx3cr1 mRNA.
• Compare anatomical localization of gene expression signals in the arcuate nucleus and ventromedial hypothalamus.
• Assess methodological controls for molecular biology studies in neuroendocrinology.

Strengths

• Includes control images (sense probe) demonstrating specificity for the in situ hybridization signals.
• Provides explicit probe sequences for the HCR method, which is a key methodological detail.
• Released under a CC-BY-4.0 license, allowing for broad reuse.

Limitations

• The dataset is a 31.5 MB PDF, which may require extraction or manual processing to access individual images and tables.
• Row count and column-level documentation are unknown, limiting automated suitability assessment.
• The description is specific to a single study's supplementary materials; generalizability to other contexts is unclear.

Provenance

Source

figshare

Collection Method

Generated as supplementary material for a published research study.

Freshness

Last updated 2026-05-31 14:48:16