Quartet RNA-seq Benchmarking Data: Isoform Expression and Splicing Profiles from 42 Labs

42 real-world laboratories contributed isoform expression and alternative splicing quantification data for the Quartet reference materials. The 3.1 GB dataset, authored by Duo Wang and last updated in April 2026, includes files for isoform FPKM and event PSI values. It is supplemented by junction reference datasets generated using long-read sequencing technology.

Use Cases

• Benchmarking RNA-seq analysis pipelines based on isoform expression (FPKM) data from 42 labs.
• Evaluating alternative splicing detection accuracy based on Percent Spliced In (PSI) values.
• Comparing laboratory-to-laboratory variation in transcript quantification based on the multi-lab study design.
• Validating novel junction predictions against long-read sequencing reference datasets mentioned in the description.

Strengths

• Data originates from a multi-institutional study involving 42 real-world laboratories.
• Includes ground truth reference datasets for junctions generated with long-read sequencing.
• Dataset size is 3.1 GB, indicating substantial content.
• Released under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Analyzed using STAR_StringTie and STAR_StringTie_SUPPA2 pipelines across multiple labs.

Time Range

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Freshness

Last updated 2026-04-15 06:21:26; freshness should be verified.

Geography

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