3D Human Liver Model for Acute and Chronic Drug-Induced Injury

This dataset contains a 3D bioprinted human liver model designed to study acute and chronic drug-induced hepatotoxicity and fibrosis. It includes encapsulated human hepatocytes and hepatic stellate cells within a hydrogel matrix, maintained over 30 days with physiological functions like albumin secretion and CYP450 activity. The model simulates low-dose long-term and high-dose short-term damage, was used to investigate fibrosis progression and immune response with incorporated monocytes. Data supports drug safety screening and chronic liver disease modeling.

Use Cases

• Evaluate drug-induced liver injury over chronic timeframes
• Study fibrosis mechanisms in a 3D multicellular human model
• Test anti-fibrotic therapies like dexamethasone

Strengths

• Long-term 30-day culture maintaining hepatocyte function
• Tri-culture design with immune cells (monocytes) enabling immune response studies
• Demonstrated reversal of fibrotic markers post-treatment

Limitations

• Specific row/column counts and file structure not detailed in description
• Data freshness unknown beyond 2024-05-10 upload
• Requires bioinformatics preprocessing for granular analysis

Provenance

Source

figshare

Collection Method

Experimental data from 3D bioprinted liver constructs, likely including RNA-seq, histology, and functional assay outputs

Time Range

Not specified, but experimental timeline spans acute and chronic injury phases up to 30 days

Geography

Lab-based study, no specific geographic scope