Aerococcus Urinae pCF10 Plasmid Transfer Data via SPI-WT Regulation

A newly discovered Aerococcus urinae strain mediates intergeneric transfer of the pCF10 plasmid, challenging the species-specific constraint of pheromone-regulated horizontal gene transfer. The dataset includes a transfer frequency of (3.41 ± 0.26) × 10⁻³ in Ae1, increasing to (7.97 ± 1.77) × 10⁻³ with exogenous cCF10 signal peptide. Authored by Man Zhang and uploaded to figshare in May 2026, this 3.5 MB document provides insights into the dissemination pathways of intestinal antibiotic resistance genes.

Use Cases

• Study intergeneric plasmid transfer frequencies based on the reported transfer rate data.
• Investigate the regulatory role of signal peptides like cCF10 and SPI-WT based on the described experimental results.
• Model the potential for antibiotic resistance gene dissemination across bacterial genera based on the described pheromone-regulated transfer mechanism.
• Analyze the function of the prgZ/prgX pathway in non-enterococcal bacteria based on the preliminary evidence provided.

Strengths

• Includes specific, quantified plasmid transfer frequencies: (3.41 ± 0.26) × 10⁻³ and (7.97 ± 1.77) × 10⁻³.
• Released under a permissive CC-BY-4.0 license, facilitating reuse.
• Focuses on a novel finding challenging the conventional understanding of pCF10 plasmid host range.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The description notes a lack of direct proof for natural secretion, binding, uptake, and transcriptional repression relief.

Provenance

Source

figshare, authored by Man Zhang.

Collection Method

Data likely originates from laboratory isolation, screening, and experimental confirmation of plasmid transfer between Aerococcus urinae Ae1 and Enterococcus faecalis.

Freshness

Last updated 2026-05-20 05:47:31; freshness should be verified.