Supplementary file 1_A universal fluorescent sensing platform based on “signal switching i

Guojing Liu uploaded a 6.9 MB DOCX file to figshare on 2026-04-21. The file describes a methodological study for high-throughput screening of xanthine oxidase inhibitors from Traditional Chinese Medicine. The research identified four inhibitors from Nelumbinis Folium extract, which contributed 61.22% of the total inhibition activity.

Use Cases

• Developing high-throughput screening assays based on the described fluorescent sensing platform.
• Identifying potential xanthine oxidase inhibitors from complex Traditional Chinese Medicine extracts.
• Validating the 'isolation-detection-identification' platform workflow for bioactive metabolite discovery.
• Studying the combined inhibitory effects of compounds like Quercetin-3-sambubioside and Hyperoside.

Strengths

• The described method offers interference-resistant detection with a high emission wavelength (λem = 690 nm) and a large Stokes shift (Δλ = 148 nm).
• The study successfully identified four specific xanthine oxidase inhibitors from Nelumbinis Folium extract.
• The combined inhibitory contribution of the identified compounds was quantified at 61.22% of the total activity.

Limitations

• The dataset is a single 6.9 MB DOCX file; the underlying raw screening data or column-level details are not provided.
• Row count and specific data structure are unknown, which limits suitability assessment for direct computational analysis.
• Last updated 2026-04-21 05:20:05; freshness should be verified as the date is in the future.

Provenance

Source

Guojing Liu via figshare.

Collection Method

The description details a research method involving a fluorescent sensing platform integrated with HPLC-FC and UHPLC-Q-TOF/MS.

Freshness

Last updated 2026-04-21 05:20:05.