Aldosterone Effects on SERCA2a and SERCA2b in Rat Mesenteric Artery Smooth Muscle Cells

A 766.9 KB supplementary document from figshare, authored by Cristian J. Arriero-Carrillo and last updated in May 2026. It details a study on how the hormone aldosterone regulates the expression and subcellular localization of calcium-handling proteins (SERCA2a and SERCA2b) in rat resistance-sized mesenteric arteries.

Use Cases

• Analyze the transcriptional regulation of SERCA2 isoforms based on mRNA and protein level changes described.
• Study subcellular protein localization shifts based on the described perinuclear and subplasmalemmal distribution patterns.
• Model calcium signaling dynamics (sparks and waves) based on the described effects of aldosterone on sarcoplasmic reticulum load.
• Investigate potential therapeutic targets for hypertension based on the described link between aldosterone, SERCA, and hyperaldosteronism.

Strengths

• Detailed biological mechanism described, including specific hormone concentration (10 nM aldosterone) and treatment duration (24 hours).
• Study includes multiple analysis levels: protein/mRNA expression, subcellular localization, and functional calcium signals.
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• The dataset is a single 766.9 KB DOCX file; the underlying experimental data (e.g., numerical measurements, images) is not directly accessible.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

figshare

Collection Method

Experimental study on rat mesenteric arteries, likely involving molecular biology and imaging techniques as described.

Time Range

Experimental timeframe not specified; publication date is 2026.

Freshness

Last updated 2026-05-04 04:24:24; freshness should be verified.

Geography

Study conducted on rat tissue; specific laboratory location is unknown.