AZTCT: Molecular Mechanisms in Recurrent Spontaneous Abortion

Supplementary data from a study investigating how Anzi Tiaochong Tang (AZTCT) inhibits ferroptosis in trophoblast cells via the METTL14/m6A/YTHDF2/SLC39A14 axis. The research combined in vivo RSA rat models with in vitro experiments on HTR-8/SVneo cells, using techniques like MeRIP and dual-luciferase assays. Findings suggest a novel epigenetic pathway for potential RSA treatment.

Use Cases

• Validating the METTL14/m6A/SLC39A14 regulatory axis in trophoblast cell models based on described experimental results.
• Comparing bioinformatics analysis of m6A methylation with wet-lab validation data from MeRIP and luciferase assays.
• Investigating ferroptosis-related gene expression changes in the context of recurrent spontaneous abortion.
• Designing follow-up studies on traditional medicine compounds like Anzi Tiaochong Tang for reproductive disorders.

Strengths

• Dataset is openly licensed under CC-BY-4.0, facilitating reuse and distribution.
• Research methodology is described in detail, including in vivo rat models and multiple in vitro validation assays.
• Cross-platform presence on figshare indicates established sharing and potential for discovery.

Limitations

• The dataset's structure is unclear; columns and row counts are not specified, hindering direct analysis.
• The primary file is a 277173-byte PDF, suggesting it contains supplementary figures/tables rather than raw, machine-readable data.
• Metadata is sparse, with no information on the author's organization or the specific temporal scope of the underlying experiments.

Provenance

Source

Suning Huang

Collection Method

Experimental research involving RSA rat models, human trophoblast cell culture (HTR-8/SVneo), bioinformatics analysis, methylated RNA immunoprecipitation (MeRIP), and dual-luciferase reporter assays.

Freshness

Last updated on 2026-04-29.