Supplementary file 1_Astragaloside IV modulates oxidative stress and osteoimmune–Wnt signa

A 6.7 MB supplementary document from a study on postmenopausal osteoporosis in rats, authored by Ya-Qing Li and last updated in April 2026. The data includes results from RNA sequencing, molecular docking, and experimental validation of Astragaloside IV treatment in ovariectomized rats. It is shared under a CC-BY-4.0 license on figshare.

Use Cases

• Identify hub genes related to osteoimmune and Wnt signaling based on RNA-seq and WGCNA results mentioned in the description.
• Validate molecular docking predictions for Astragaloside IV interactions with targets like Lep, Ptgs2, and Wnt1.
• Correlate serum bone-turnover markers (CTX-I, OCN, PTH) with micro-CT bone structure parameters (BMD, BV/TV).
• Analyze multiplex immunofluorescence data for protein expression (NF-κB p65, Wnt1, Gfap) in femoral trabecular bone.

Strengths

• Includes multiple experimental modalities: RNA sequencing, molecular docking, ELISA, micro-CT, and multiplex immunofluorescence.
• Data is associated with a specific, controlled animal model (ovariectomized Sprague-Dawley rats) and treatment protocol (AS-IV at 20/40/80 mg/kg).
• Shared under a permissive CC-BY-4.0 license, facilitating reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The primary data file is a DOCX document, which may require extraction or conversion for computational analysis.

Provenance

Source

figshare

Collection Method

Data generated from a controlled laboratory study involving ovariectomized rats, RNA sequencing, and molecular docking.

Time Range

The experimental timeline involved ovariectomy, a 6-week wait, and an 8-week treatment period, but the exact dates of data collection are not specified.

Freshness

Last updated 2026-04 13 05:47:33; freshness should be verified.

Geography

Laboratory study; geographic location of the research institution is unknown.