Single-Cell RNA Sequencing of B Cells in Antisynthetase Syndrome

Peripheral blood B cells from patients with antisynthetase syndrome and healthy controls were analyzed. The dataset contains single-cell RNA sequencing results for flow-sorted CD19+ cells, identifying dysregulated pathways. It was authored by Erin M. Wilfong and last updated in April 2026.

Use Cases

• Identify differential gene expression patterns in activated versus memory B cell subsets from ASyS patients.
• Analyze upregulated pathways related to interferon response, cellular stress, and actin cytoskeleton rearrangement in specific B cell populations.
• Correlate expression of specific stress response genes like FKBP5 or MYADM with clinical disease status in antisynthetase syndrome.
• Investigate gene signatures associated with antigen presentation and cellular adhesion in MYADM+ memory B cells.

Strengths

• Single-cell resolution provides granular data on B cell heterogeneity.
• Analysis includes a direct comparison between ASyS patients and healthy controls.
• Dataset is under an open CC-BY-4.0 license, facilitating reuse.

Limitations

• The dataset is small at 4.1 MB, indicating limited raw sequencing data or a focused analysis file.
• Sample size (number of patients and cells) is not specified, limiting assessment of statistical power.
• Data is provided as a DOCX document, which is not a standard bioinformatics format for raw or processed sequencing data.

Provenance

Source

Figshare, authored by Erin M. Wilfong.

Collection Method

Single-cell RNA sequencing performed on flow-sorted CD19+ B cells from peripheral blood mononuclear cells.

Freshness

Last updated in April 2026, indicating it is a forward-dated or planned future release.