Gucy1a3 Knockout Mouse Cerebrovascular Analysis for Moyamoya Disease Research

A supplementary research document detailing cerebrovascular phenotype analysis in Gucy1a3 loss-of-function mice. The study, authored by Pingkai Wang and last updated in April 2026, investigates the relationship between GUCY1A3 deficiency and moyamoya disease susceptibility using high-resolution MRA, vascular casting, and histopathology. Results include quantified metrics for vascular branching, density, and microvessel caliber in knockout versus wild-type mice.

Use Cases

• Validate genetic associations for moyamoya disease based on described animal model phenotypes.
• Analyze leptomeningeal vascular network topology based on skeletonization and branching data mentioned in the description.
• Compare cortical microvascular density and caliber between genotypes based on reported CD31 immunohistochemistry results.
• Investigate the relationship between soluble guanylate cyclase function and small-vessel integrity based on the study's conclusions.

Strengths

• Includes specific, statistically significant quantitative results (e.g., vascular branching 365.90 ± 14.92 vs. 330.00 ± 9.72).
• Employs multiple, detailed methodological approaches (7.0T MRA, vascular casting, H&E/EVG staining, immunohistochemistry).
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• The primary data file is a 279.1 KB DOCX document, which is a small supplementary file rather than a raw dataset.
• Column-level documentation and sample data are unavailable; data semantics must be inferred from the text.
• Row count is unknown, which may limit suitability assessment for computational analysis.

Provenance

Source

figshare, author Pingkai Wang.

Collection Method

Experimental study using Gucy1a3−/− and wild-type mice, with data gathered via magnetic resonance angiography, vascular casting, and histopathological staining.

Time Range

Study conducted on early adult (8–12 weeks) mice.

Freshness

Last updated 2026-04-13 04:35:50.

Geography

Laboratory study; geographic coverage is not specified.