Neutrophil Subsets in Primary Sjögren’s Syndrome from Single-Cell RNA Sequencing

Single-cell RNA sequencing data from peripheral blood neutrophils of patients with primary Sjögren’s syndrome and healthy controls. The dataset, shared by Zhaomeng Gao on figshare, identifies six neutrophil subsets and their roles in disease progression. Analysis includes differential gene expression, pathway enrichment, and cell-cell interaction mapping.

Use Cases

• Identifying neutrophil subset biomarkers for Sjögren’s syndrome based on the six clustered subsets described.
• Analyzing cell-cell interaction pathways in autoimmune disease based on the described CCL5-CCR1 and LGALS9-HAVCR2 interactions.
• Validating potential therapeutic targets like LY6E and CLEC12A based on the differentially expressed genes highlighted.
• Studying neutrophil differentiation trajectories based on the pseudo-time analysis from Neutrophil 2 to Neutrophil 4.

Strengths

• Includes data from both newly diagnosed pSS patients and healthy controls, enabling comparative analysis.
• Analysis covers multiple techniques: scRNA-seq clustering, GO/KEGG enrichment, and RT-qPCR validation.
• Dataset is openly shared under a CC-BY-4.0 license, facilitating reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect temporal and geographic bias inherent to collection from a single hospital in Ningxia between December 2022 and January 2025.

Provenance

Source

Rheumatology and Immunology Department of Ningxia Medical University General Hospital.

Collection Method

Single-cell RNA sequencing of peripheral blood neutrophils from patients and controls, followed by bioinformatics analysis.

Time Range

Samples collected between December 2022 and January 2025.

Freshness

Last updated 2026-05-04 05:30:17; freshness should be verified.

Geography

Likely Ningxia, China, based on the hospital location.