Characterization of Lytic Salmonella Phage GF04 and Receptor Mutation Analysis

A 1.4 MB supplementary document details the isolation and characterization of Salmonella phage GF04. The research includes adsorption kinetics, a one-step growth curve with a burst size of ~103 PFU, and host range analysis on 19 clinical isolates. Authored by Gabriel H. Fares and last updated in May 2026, the file is shared under a CC-BY-4.0 license.

Use Cases

• Studying phage adsorption kinetics and replication efficiency based on the described one-step growth curve analysis.
• Identifying genetic determinants of phage resistance based on the comparative genomic analysis of a spontaneous resistant variant.
• Assessing the therapeutic potential of a lytic phage based on host range data across 19 Salmonella isolates.
• Modeling structural effects of receptor mutations based on the use of AlphaFold to analyze the yjiK_2 P128L mutation.

Strengths

• The dataset includes specific quantitative results, such as a burst size of ~103 PFU per infected cell and adsorption within 5 minutes.
• Genomic analysis confirmed the absence of lysogeny, virulence, or antimicrobial resistance genes, which is a key safety consideration for therapeutic use.
• The host range is tested against 19 clinical Salmonella isolates, providing concrete data on strain-dependent infectivity.

Limitations

• The primary data format is a DOCX document; underlying raw data files (e.g., sequence reads, raw assay data) are not specified.
• Column-level documentation is absent; field semantics must be inferred from the textual description.
• Row count is unknown, which may limit suitability assessment for structured data analysis.

Provenance

Source

figshare

Collection Method

Phage GF04 was isolated from sewage using a specific Salmonella host strain and characterized through plaque assays, growth curves, and whole genome sequencing.

Freshness

Last updated 2026-05-14 05:49:52; freshness should be verified.