Multi-Omics Analysis of Blood-Brain Barrier Disruption in Tuberculous Meningitis

A 16.4 KB supplementary document details a multi-omics study of tuberculous meningitis. The research, authored by Mengyu Luan and last updated in June 2026, integrates bulk and single-cell RNA sequencing from clinical and murine datasets to investigate blood-brain barrier disruption. It identifies three significantly upregulated genes and proposes a novel mechanistic link between MARCKS and the GSDMD pyroptosis pathway.

Use Cases

• Identify potential therapeutic targets for barrier-stabilizing strategies based on the nominated genes MARCKS, CD274, and IL17RA.
• Investigate co-expression patterns between inflammatory signaling and cytoskeletal regulation genes mentioned in the description.
• Validate findings from murine models in human tissue, as suggested by the description's call for further validation.

Strengths

• The study employs an integrated multi-omics approach combining bulk and single-cell RNA sequencing.
• It identifies and validates three specific genes (MARCKS, CD274, IL17RA) as significantly upregulated in TBM.
• The document is openly licensed under CC-BY-4.0 for reuse.

Limitations

• The dataset is a 16.4 KB DOCX file, indicating limited scope and likely containing summary text rather than raw data tables.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for direct analysis.

Provenance

Source

figshare

Collection Method

Integrated multi-omics analysis combining bulk and single-cell RNA sequencing of clinical and murine datasets with experimental validation.

Freshness

Last updated 2026-06-03 05:43:02; freshness should be verified.