Comparative Proteomic Analysis of Colorectal Cancer Stem Cells from Two Cell Lines

A 2.1 MB supplementary file contains proteomic data comparing cancer stem cell-enriched spheroids from SW620 and HCT-116 colorectal cancer cell lines to their parental adherent cells. The data was generated using mass spectrometry-based label-free shotgun proteomics and analyzed with Ingenuity Pathway Analysis. The dataset, authored by Ola J. Hussein and last updated on 2026-04-20, is shared under a CC-BY-4.0 license.

Use Cases

• Identify potential protein biomarkers for colorectal cancer stem cells based on differentially abundant proteins.
• Analyze dysregulated canonical pathways, such as metabolic reprogramming and TGF-β signaling, associated with stemness.
• Validate predicted upstream regulators like MYC, MLXIPL, and HIF-related signaling using the provided proteomic profiles.
• Investigate the role of specific proteins like HMGCS1 in the mevalonate pathway within cancer stem cell contexts.

Strengths

• Data is derived from a comparative proteomic study of two established colorectal cancer cell lines (SW620 and HCT-116).
• Analysis includes validation of selected findings via RT-qPCR and/or Western blotting, as mentioned in the description.
• Shared under a permissive CC-BY-4.0 license, facilitating reuse and redistribution.

Limitations

• The dataset is a 2.1 MB DOCX file; the underlying tabular data structure and column definitions are not directly visible.
• Row count and specific column-level documentation are unknown, requiring manual inspection after download.
• Data is limited to in vitro cell line models, which may not fully represent in vivo tumor heterogeneity.

Provenance

Source

figshare

Collection Method

Mass spectrometry-based label-free shotgun proteomics performed on CSC-enriched spheroids and parental adherent cells.

Freshness

Last updated 2026-04-20 04:13:40; freshness should be verified for current research.