Spinal Cord Injury Transcriptomics: m6A Regulators and Immune Cell Infiltration

Transcriptomic datasets from the Gene Expression Omnibus (GEO) were analyzed to identify 14 differentially expressed m6A RNA methylation regulators in spinal cord injury. The research, authored by Xiaoqin Liu and last updated in May 2026, used machine learning to identify hub genes FTO and YTHDC1 and validated them in an independent dataset and a rat model. The analysis includes immune cell infiltration profiles and single-cell RNA sequencing data.

Use Cases

• Identifying potential biomarkers for spinal cord injury based on the expression of m6A regulators like FTO and YTHDC1.
• Analyzing immune microenvironment changes in spinal cord injury using single-sample gene set enrichment analysis (ssGSEA) results.
• Constructing miRNA–gene–TF interaction networks for spinal cord injury pathogenesis using the described methodology.
• Validating gene expression patterns in specific cell types like microglia and astrocytes using the referenced single-cell RNA sequencing data.

Strengths

• Analysis is based on transcriptomic datasets from the authoritative Gene Expression Omnibus (GEO) repository.
• Findings were validated through multiple methods, including an independent dataset, single-cell RNA sequencing, and in vivo rat model experiments.
• The dataset is openly shared under a CC-BY-4.0 license, facilitating reuse.

Limitations

• The primary data file is a 74.4 KB DOCX document, which likely contains summarized results rather than the raw underlying transcriptomic data.
• Row count and column-level documentation for any underlying data tables are unknown, limiting suitability assessment.
• Description metadata is limited; actual data quality and structure require manual inspection after download.

Provenance

Source

Gene Expression Omnibus (GEO)

Collection Method

Transcriptomic data analysis using machine learning algorithms, immune infiltration assessment, and experimental validation.

Freshness

Last updated 2026-05-20 05:46:05; freshness should be verified.