Rhizoctonia solani Diagnostic Marker Validation Data for Rice Plants and Soil

Supplementary data accompanies a study developing a molecular marker for detecting the rice sheath blight pathogen Rhizoctonia solani AG1-IA. The data likely contains results from PCR, qPCR, and LAMP assays used to validate the marker's sensitivity and specificity in infected rice plants and soil samples. The dataset was authored by Asmita Singh and last updated on May 21, 2026.

Use Cases

• Benchmarking molecular assay sensitivity based on the reported detection limits of 100 pg (PCR) and 10 pg (qPCR)
• Evaluating pathogen detection in soil samples based on the description of validation in rice-wheat cropping system soils
• Comparing disease resistance in rice varieties based on the reported copy number differences between susceptible and resistant genotypes
• Developing field-deployable diagnostic kits based on the described LAMP assay optimized for visual detection

Strengths

• Assays were validated across multiple rice genotypes, suggesting broad applicability
• The qPCR assay demonstrated high sensitivity (10 pg DNA) and accuracy (R² > 0.99)
• The LAMP assay provided rapid visual detection with reported specificity against other rice pathogens

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The data is contained in a DOCX file (693.9 KB), which is a small and non-standard format for structured data analysis

Provenance

Source

figshare

Collection Method

Likely contains experimental results from laboratory assays (PCR, qPCR, LAMP) performed on infected rice plants and soil samples.

Freshness

Last updated 2026-05-21 05:42:23; freshness should be verified