Tomato Genome Editing Protocol with Agroinfiltration Efficiency Results

A PDF protocol describes an in planta genome editing method for tomatoes, developed by Misaki Kobayashi. The study combines transient expression of Cas9, guide RNAs, and developmental regulators via agroinfiltration to avoid stable transfection and tissue culture. Chimeric mutants were obtained with an efficiency of 11.7%, and most observed mutations were single base substitutions.

Use Cases

• Benchmarking transient expression genome editing protocols based on the described agroinfiltration method.
• Analyzing mutation efficiency and types based on the reported 11.7% success rate and prevalence of single base substitutions.
• Developing tissue culture-free editing workflows for crops based on the in planta method described.

Strengths

• The protocol reports a specific mutagenesis efficiency of 11.7% for the method.
• The description details the specific components used: Cas9, guide RNAs, and developmental regulators.
• The file is licensed under CC-BY-4.0, allowing for reuse.

Limitations

• The dataset is a 1.0 MB PDF file; the underlying experimental data (e.g., raw sequences) is not provided.
• Column-level documentation is absent; field semantics must be inferred from the text.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

figshare

Collection Method

The data likely contains the experimental protocol and results from a research study.

Freshness

Last updated 2026-04-09 04:10:15; freshness should be verified.