Supplementary file 1_Dissecting the disconnect between circuit activation and dominant ada

Jie-Ning Chuang's supplementary document from 2026 details an experiment on cytoplasmic phage-assisted continuous evolution (PACE) of a 7D12 nanobody targeting EGFR DIII. The 3.4 MB DOCX file contains results on circuit activation, phage propagation, and mutational diversity under drift conditions. It was published on figshare under a CC-BY-4.0 license.

Use Cases

• Analyzing the decoupling between circuit activation and adaptive evolution based on experimental results described in the document.
• Studying constraints on cytoplasmic evolution of mammalian proteins based on described biochemical limitations like disulfide bonds.
• Investigating mutational convergence and interface accessibility in nanobody binding based on structural analysis and predicted saturation mutagenesis mentioned.
• Evaluating the performance of non-continuous flow (PANCE) and PACE methods under extended drift conditions as described in the results.

Strengths

• The dataset is a 3.4 MB supplementary document providing detailed experimental methodology and results.
• The document likely contains specific findings on soluble fraction retention and pairing activity of nanobodies and EGFR DIII in the cytoplasm.
• Results include analysis of circuit activation, phage propagation, and mutational diversity under specific selection pressures.

Limitations

• The primary data format is a DOCX document; underlying raw data tables or sequences are not directly accessible.
• Row count and column-level documentation are unknown, which limits suitability assessment for computational analysis.
• Description metadata is limited; actual data quality and structure require manual inspection after download.

Provenance

Source

figshare, authored by Jie-Ning Chuang.

Collection Method

Likely contains experimental data and analysis from a study on cytoplasmic phage-assisted continuous evolution (PACE).

Freshness

Last updated 2026-05-29 06:11:36.