Syndecan-4 Roles in Chicken Fibrosis In Vitro Study

A research document investigates the dual roles of syndecan-4 (SDC4) in regulating fibrosis within broiler chicken fibroblasts, related to Wooden Breast myopathy. The study uses an in vitro overexpression system and blocking peptides to analyze gene and protein expression of fibrotic markers like collagen I, collagen III, and MMP-2. The file is 7.4 MB and was authored by Lucie Pejšková under a CC BY 4.0 license.

Use Cases

• Analyze the relationship between SDC4 overexpression and changes in gene expression for fibrotic markers like collagen I, collagen III, and MMP-2.
• Investigate the modulation of SDC4 ectodomain shedding using blocking peptides targeting specific amino acid regions (76–106 and 100–130).
• Examine the effect of TGF-β1 stimulation on the production of the 20 kDa SDC4 fragment and fibrotic marker gene expression.
• Study the phosphorylation levels of intracellular signaling proteins p38 MAPK and Akt under SDC4 overexpression conditions.

Strengths

• The study provides detailed molecular data on SDC4 and its fragments (15 kDa and 20 kDa) in a specific biological context.
• Research focuses on a defined agricultural pathology (Wooden Breast myopathy) using controlled in vitro methods.
• The dataset is openly available under a permissive CC BY 4.0 license.

Limitations

• The primary data is embedded within a narrative DOCX file, requiring extraction for quantitative analysis.
• The dataset is small (7.4 MB) and contains textual results rather than a structured, machine-readable table.
• Experimental scope is limited to in vitro chicken fibroblast models, which may not fully capture in vivo complexity.

Provenance

Source

figshare, authored by Lucie Pejšková.

Collection Method

In vitro experiments using chicken fibroblasts, including SDC4 overexpression, blocking peptide treatment, and TGF-β1 stimulation.

Time Range

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Freshness

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Geography

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