Ectoine Anti-Senescence Effects on Human Keratinocytes and Endothelial Cells

Supplementary file 1 from a study evaluating the anti-cellular senescence effect of Ectoine on H2O2-induced oxidative stress in HaCaT and EA.hy926 cells. The dataset includes results from CCK-8, EdU, SA-β-gal staining, ROS measurement, flow cytometry, qRT‒PCR, and Western blot assays. The file was authored by Meini Li and last updated on 2026-03-18.

Use Cases

• Analyze the concentration-dependent effect of Ectoine on cell viability based on CCK-8 assay results.
• Investigate the modulation of p53/p21 and p16 pathways based on qRT‒PCR and Western blot data for TP53, CDKN1A, and CDKN2A.
• Study the correlation between ROS levels and senescence markers based on DCFH probe and SA-β-gal staining results.
• Evaluate changes in proliferative capacity and cell death based on EdU assay and flow cytometry data.

Strengths

• Data is associated with a detailed methodological description covering multiple in vitro assays.
• The file is licensed under CC-BY-4.0, permitting open sharing and adaptation.
• The most effective concentration of Ectoine (0.50 μmol/L) is explicitly identified in the results.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The primary data format is a DOCX file, which may require parsing to extract structured data.

Provenance

Source

figshare, author Meini Li

Collection Method

Data generated through in vitro laboratory assays on HaCaT and EA.hy926 cell lines.

Freshness

Last updated 2026-03-18 07:45:38; freshness should be verified.