Mouse Model of Thermal-Induced Spinal Cord Injury with Transcriptomic Analysis

A 452.3 KB PDF file by Arata Mashima describes a novel mouse model for thermal-induced spinal cord injury. The model applies 90°C heat for 1 minute to the thoracic cord, resulting in persistent motor deficits, demyelination, and collagen deposition. Transcriptomic analysis via RNA-sequencing identified upregulated pro-fibrotic genes and implicated specific pathways in scar progression.

Use Cases

• Study the pathophysiology of thermal damage in spinal surgery based on the described experimental model.
• Analyze gene expression changes related to fibrosis based on the mentioned upregulation of Col1a1, Col1a2, Tgfβ1, and Acta2.
• Investigate the role of Wnt/β-catenin signaling in fibrotic scar progression based on the late induction of Fzd7.
• Examine the temporal dynamics of immune response in spinal injury based on the early upregulation of Scarb3 and Actg2 versus later Itgax.

Strengths

• The experimental protocol is precisely defined, using a controlled thermal exposure of 90°C for 1 minute.
• Transcriptomic analysis via RNA-sequencing provides molecular insights into the injury response.
• The study uses Col1a2-EGFP transgenic mice to visualize Type I collagen-producing cells at the lesion site.

Limitations

• The dataset is a single 452.3 KB PDF file, which is a very limited data scope.
• Column-level documentation for any underlying raw data is absent; field semantics must be inferred from the paper.
• Row count for any potential underlying data is unknown, which may limit suitability assessment.

Provenance

Source

figshare, authored by Arata Mashima.

Collection Method

Experimental data generated from a controlled laboratory study on mice.

Freshness

Last updated 2026-03-17 17:12:56.