In Vitro Study of 4-Aminopyridine and Cisplatin Ototoxicity

A collection of experimental results from an in vitro study evaluating the otoprotective potential of 4-aminopyridine against cisplatin-induced ototoxicity. The study used mouse cochlear explants and HeLa cells, with measurements including caspase 3/7 activity, cisplatin-DNA adducts, and cleaved caspase-3 staining. The file is a 225.3 KB DOCX document authored by Sofia Waissbluth and last updated in March 2026.

Use Cases

• Analyze caspase 3/7 activity data from cochlear explants treated with cisplatin and 4-aminopyridine.
• Compare cisplatin-DNA adduct staining levels between HeLa cells and cochlear explants under different treatment conditions.
• Examine cleaved caspase-3 immunohistochemistry results to assess cell death in the in vitro models.

Strengths

• Data is derived from controlled in vitro experiments using mouse cochlear explants and HeLa cells.
• The dataset is associated with a specific, peer-reviewed research hypothesis on OCT2 and MATE1 transport mechanisms.
• The file is under a permissive CC BY 4.0 license for reuse.

Limitations

• The dataset is very small, consisting of a single 225.3 KB DOCX file, limiting computational analysis scope.
• The specific data structure, column names, and row counts are not provided, complicating direct data extraction.
• The study concluded no observed otoprotective effect, which may limit its utility for certain predictive modeling tasks.

Provenance

Source

figshare, authored by Sofia Waissbluth.

Collection Method

Experimental data from in vitro treatment of mouse cochlear explants and HeLa cells with cisplatin and 4-aminopyridine, followed by caspase activity assays and immunohistochemistry.

Freshness

Last updated on 2026-03-20.