Bacteriorhodopsin Production and Characterization from Hypersaline Salt Pan Haloarchaea

A supplementary document details the extraction and characterization of bacteriorhodopsin from haloarchaeal strains isolated from the Tuticorin salt pan. The study reports a production yield of 360 mg/L from Halostagnicola larsenii (TP6) and describes methods including SDS-PAGE, TLC, R-HPLC, and Raman spectroscopy. The file was authored by Steffy Joseph and last updated in June 2026.

Use Cases

• Developing photoelectrochemical biosensors based on the described bacteriorhodopsin's light-driven proton pumping activity.
• Optimizing biomolecule production from extremophilic microbes using the described extraction and characterization methods.
• Assessing the feasibility of bacteriorhodopsin for optical devices based on its reported photochemical stability and energy conversion properties.

Strengths

• Reports a specific, high bacteriorhodopsin production yield of 360 mg/L from a native strain.
• Describes multiple characterization methods (SDS-PAGE, TLC, R-HPLC, Raman spectroscopy) applied to the extracted protein.
• Includes a successful prototype biosensor development, demonstrating applied research outcomes.

Limitations

• The dataset is a 989.4 KB DOCX file, suggesting limited scope and primarily textual/documentary content.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

figshare

Collection Method

Bacteriorhodopsin was extracted from haloarchaeal strains isolated from the Tuticorin salt pan using the Bead mix method.

Freshness

Last updated 2026-06-04 05:21:10; freshness should be verified.

Geography

Tuticorin salt pan