Supplementary file 1_Faecal inflammatory protein markers in children with autism spectrum

57 children with autism spectrum disorder (ASD) and 57 of their healthy biological siblings were compared for fecal levels of α1-antitrypsin, immunoglobulin A, and calprotectin. The dataset, authored by Joško Osredkar and last updated in April 2026, contains results from a sibling-controlled study designed to isolate ASD-specific biology from shared environmental factors. No statistically significant differences in inflammatory markers were found between the groups.

Use Cases

• Compare gut inflammatory protein levels between ASD and control groups based on sibling-pair design
• Analyze correlations between biomarker levels and ASD severity based on Childhood Autism Rating Scale (CARS) scores
• Investigate subgroup patterns in moderate versus severe ASD based on exploratory IgA and S100A8/S100A9 ratio findings
• Validate or challenge hypotheses about gut inflammation in ASD based on quantified fecal protein data

Strengths

• Sibling-controlled design with 57 matched pairs, which helps control for shared environmental and microbiome factors
• Participants were screened to exclude recent antibiotic use, digestive problems, and infections, reducing confounding variables
• Proteins were quantified using UHPLC–MS/MS with stable isotope–labelled peptides and internal standards for precise measurement

Limitations

• Row count is unknown, which may limit suitability assessment
• Subgroup analyses for severe ASD were critically underpowered (n=11 pairs) and are described as hypothesis-generating only
• Column-level documentation is absent; field semantics must be inferred after download

Provenance

Source

figshare

Collection Method

Stool samples were collected, processed via freeze-drying and protein extraction, and analyzed by UHPLC–MS/MS.

Time Range

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Freshness

Last updated 2026-04-15 09:17:44; freshness should be verified

Geography

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