LCN2-Deficient PC-3 Cells: Functional and Genomic Characterization

A supplementary document from a 2026 study by Kiara Gäberlein, published on figshare. It details the functional and molecular effects of depleting the lipocalin 2 (LCN2) gene in an aggressive prostate cancer cell model. The analysis includes STR authentication, next-generation sequencing, RT-qPCR validation, and spectral karyotyping of the CRISPR/Cas9-edited cell line.

Use Cases

• Study the role of LCN2 in prostate cancer cell invasiveness and cytoskeletal organization based on the described functional assays.
• Analyze genomic changes resulting from CRISPR/Cas9 editing in a cancer cell line based on the described sequencing and karyotyping methods.
• Investigate links between LCN2, inflammation, and endoplasmic reticulum stress in cancer based on the pathways mentioned in the results.

Strengths

• Data is associated with a specific, authenticated cell line (PC-3) and a genetically modified clone (PC-3 LCN2-KO#1).
• Analysis employs multiple validation techniques, including STR analysis, next-generation sequencing, RT-qPCR, and spectral karyotyping.
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• The primary data file is a 1.1 MB DOCX document; underlying raw sequencing or quantitative data may not be directly accessible.
• Row and column structures are unknown, as the dataset consists of a supplementary document rather than a structured data table.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Genetic analysis of parental and CRISPR/Cas9-edited prostate cancer cell lines, including STR analysis, next-generation sequencing, and RT-qPCR.

Freshness

Last updated 2026-04-20 09:07:39; freshness should be verified.