iVero.219-mcRTA: Supplementary Data on a Doxycycline-Inducible KSHV Producer Cell Line

Li Wang's supplementary file from 2026 details the generation of the iVero.219-mcRTA cell line for Kaposi’s sarcoma-associated herpesvirus (KSHV) research. The document reports quantitative performance metrics, including a viral DNA copy number of (5.7 ± 0.1) × 10⁴ genome copies/mL and an infectious titer of (2.4 ± 0.1) × 10⁴ IU/mL upon doxycycline induction. This 254.0 KB document is licensed under CC-BY-4.0 and was last updated on 2026-04 13.

Use Cases

• Benchmarking viral production titers based on reported genome copy and infectious unit measurements.
• Designing HDACi-independent viral reactivation experiments based on the described doxycycline-only induction protocol.
• Evaluating the efficiency of multicopy gene integration based on the reported ~150 RTA cassette copies per cell.
• Planning infection assays based on the Poisson distribution analysis method described for titer calculation.

Strengths

• Provides specific quantitative results, including viral DNA copies ((5.7 ± 0.1) × 10⁴/mL) and infectious titers ((2.4 ± 0.1) × 10⁴ IU/mL).
• Documents a clear methodological advance: high-titer KSHV production without histone deacetylase inhibitor co-treatment.
• Reports a high RTA cassette copy number of approximately 150 per cell, as determined by droplet digital PCR.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 254.0 KB file size suggests a limited scope, likely containing summary data rather than raw experimental datasets.

Provenance

Source

figshare

Collection Method

Experimental generation of a polyclonal cell line via sequential lentiviral transduction, as described in the document.

Time Range

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Freshness

Last updated 2026-04-13 04:35:42; freshness should be verified.

Geography

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