Supplementary File 1: Chitinase Production and Immunomodulatory Efficacy Data

Longzhi Chen published a supplementary document on figshare on 2026-05-11 detailing the optimization and characterization of a novel chitinase enzyme. The 2.2 MB document describes a 49.43-fold enhancement in enzyme production and the immunomodulatory effects of its product, N-acetylglucosamine, in a mouse model. The data likely contains experimental results, optimization parameters, and efficacy measurements supporting the described biotechnological and immunological findings.

Use Cases

• Benchmarking chitinase production yields based on the described optimization strategies (OFAT, PBD, BBD).
• Analyzing enzyme kinetics based on the reported Km value and specific activity toward colloidal chitin.
• Studying immunomodulatory effects of N-acetylglucosamine based on the described restoration of body weight and serum markers in immunosuppressed mice.
• Evaluating enzyme stability based on the reported pH tolerance and reagent compatibility data.

Strengths

• Describes a 49.43-fold enhancement in chitinase production through sequential optimization.
• Reports specific enzyme metrics, including a Km of 0.24 mg/mL and specific activity of 7.96 U/mg.
• Validates findings in an in vivo model, showing significant restoration of immune parameters (p < 0.05).

Limitations

• The dataset is a 2.2 MB DOCX file; data structure and machine-readability are unknown.
• Column-level documentation is absent; field semantics must be inferred from the document text.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

Longzhi Chen via figshare

Collection Method

Experimental research involving enzyme isolation, optimization, purification, and animal model testing.

Freshness

Last updated 2026-05-11 04:25:57; freshness should be verified.