Human-associated NDM-5 E. coli in Hungarian Retail Meat, 2021

Three multidrug-resistant Escherichia coli isolates from retail beef and pork in Hungary were characterized in 2021. The isolates, belonging to the human-associated uropathogenic clone ST405, were analyzed using phenotypic susceptibility testing and whole-genome sequencing. Data includes genomic profiles for resistance to carbapenems, cephalosporins, fluoroquinolones, tetracycline, and azithromycin.

Use Cases

• Analyze the genomic profiles of three ST405 E. coli isolates to map resistance to carbapenems, cephalosporins, and fluoroquinolones.
• Compare the approximately 132 kb IncFII-IncFIB hybrid plasmids carrying the bla_NDM-5 gene to clinical plasmids from human isolates.
• Investigate clonal relationships and single nucleotide polymorphisms among the three characterized isolates.
• Study horizontal gene transfer potential using data from conjugation experiments performed on the isolates.

Strengths

• Three fully characterized E. coli isolates with detailed phenotypic and genotypic data.
• Hybrid genome assemblies from Illumina and Oxford Nanopore sequencing enable comprehensive plasmid analysis.
• Data supports One Health surveillance by linking retail meat isolates to human-associated clone ST405 and international clinical strains.

Limitations

• Extremely small sample size of only three bacterial isolates limits statistical power.
• Data is confined to a single country (Hungary) and year (2021), restricting geographical and temporal generalizability.
• Primary data is presented in a DOCX document, requiring extraction for computational analysis.

Provenance

Source

Mirena Ivanova, via figshare.

Collection Method

E. coli isolates from retail meat subjected to antimicrobial susceptibility testing, conjugation experiments, and whole-genome sequencing.

Time Range

2021

Freshness

Data from 2021, last updated in the repository on 2026-03-24.

Geography

Hungary