Impact of Liquid Dosing on a 3D Human Bronchial Epithelial Model for Protease Studies

A 2026 figshare document by Georgina Hopkins details a study using a human air-liquid interface bronchial epithelial model (MucilAir™) to investigate protease exposure. The work assesses the impact of liquid dosing and protease concentrations from 0.00125 to 7812 μg/mL on epithelial barrier integrity, cytokine production, and extracellular vesicle dynamics. It provides a foundation for developing in vitro methodologies for inhalation risk assessment.

Use Cases

• Benchmarking in vitro dosing strategies based on MPPD model simulations described in the study
• Investigating the impact of liquid dosing on epithelial barrier integrity measured by transepithelial electrical resistance (TEER)
• Analyzing protease effects on extracellular vesicle dynamics and cytokine secretion (e.g., IL-8)
• Developing methodology for risk assessment of inhaled enzymes based on the described experimental framework

Strengths

• Document is licensed under CC-BY-4.0, allowing for broad reuse.
• Study tests a wide concentration range of protease (0.00125–7812 μg/mL).
• The work is part of a program developing human-relevant New Approach Methodologies (NAMs).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The data is contained in a 1.4 MB DOCX file, which may require parsing to extract structured information.

Provenance

Source

figshare

Collection Method

Experimental study using a 3D human bronchial epithelial model (MucilAir™) with repeated apical liquid exposures.

Freshness

Last updated 2026-05-27 02:00:03; freshness should be verified.