Supplementary file 1_Integrated transcriptomics identifies ER stress–associated apoptosis

A supplementary research file from a study investigating Dl-3-n-butylphthalide's effects on acute kidney injury following traumatic cardiac arrest in pigs. The 29.3 MB document, authored by Kechun Zhou and last updated in June 2026, integrates bulk transcriptomic, microarray, and single-cell RNA sequencing data with in vivo validation using Bama minipigs. It prioritizes a proximal tubule-enriched ER stress-apoptosis module and associates early NBP administration with reduced kidney injury markers.

Use Cases

• Validate ER stress and apoptosis gene signatures based on integrated bulk and single-cell transcriptomics mentioned in the description.
• Benchmark drug efficacy metrics based on serum creatinine and blood urea nitrogen measurements from a porcine model.
• Study proximal tubule cell state progression during injury based on pseudotime inference and PT-state stratification analyses described.
• Investigate correlations between protein expression (PERK, CHOP, caspase-3) and histopathologic injury scores from TUNEL and H&E staining.

Strengths

• Integrates multiple high-resolution data types including bulk transcriptomics, microarray, and single-cell RNA sequencing.
• Includes in vivo validation with specific measurements like serum creatinine and blood urea nitrogen at 1, 2, 4, and 24 hours post-resuscitation.
• Provides detailed methodological description of analyses like Hallmark GSEA, GSVA/ssGSEA, and pseudo-bulk differential analysis.

Limitations

• The primary data file is a 29.3 MB DOCX document, which may contain processed results and figures rather than raw, reusable data tables.
• Row and column counts are unknown, making it difficult to assess the dataset's scale and structure for direct computational analysis.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Integrated analysis of ischemia–reperfusion injury-related transcriptomic datasets combined with an in vivo porcine model experiment.

Freshness

Last updated 2026-06-04 05:33:33; freshness should be verified.