Integrative circRNA Landscape of Intrauterine Adhesions: ceRNA Axes and Splicing Usage

A 2026 integrative analysis of circular RNA (circRNA) in intrauterine adhesions (IUA) by Yingqing Chen. The study identifies 1,724 high-confidence circRNAs from 1,143 host genes, constructs ceRNA circuits, and profiles circRNA-associated splicing usage linked to uterine contractility and immunity.

Use Cases

• Construct competing endogenous RNA (ceRNA) circuits based on identified circRNA-miRNA-mRNA interactions.
• Analyze circRNA-associated splicing usage shifts in pathways related to uterine contractility and EGFR signaling.
• Validate dysregulated circRNAs and their target mRNAs using back-splice junction-spanning RT-qPCR.
• Correlate differential circRNA expression with macrophage phenotype expansion via immune deconvolution.
• Build circRNA-RBP co-expression networks based on differentially expressed RNA-binding proteins.

Strengths

• Catalog includes 1,724 high-confidence circRNAs derived from four independent circRNA callers.
• Analysis integrates multiple methodologies: ceRNA circuit construction, immune deconvolution, and splicing usage quantification.
• Validation was performed in an independent clinical cohort using RT-qPCR and Western blotting.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a supplementary PDF file (588.5 KB), likely containing summary results rather than raw sequencing data.

Provenance

Source

figshare

Collection Method

Reanalysis of rRNA-depleted RNA sequencing data from IUA and controls (GSE224093).

Freshness

Last updated 2026-05-07 04:37:43; freshness should be verified.