Optimized VirScan Protocol for Enhanced Epitope Serology

Lily Kjendal's supplementary document details an optimized PhIP-seq workflow for VirScan viral serology. The protocol, validated across human and rabbit sera, improves signal-to-noise and enables quantitative detection of HIV epitopes across a 100-fold dilution series. It identifies strain-specific influenza hemagglutinin epitopes elicited by vaccination.

Use Cases

• Analyze enriched peptide population expansion and normalized peptide counts from iterative immunoprecipitation data.
• Map immunodominant gp160 regions and other viral epitopes resolved by the serum pre-wash protocol.
• Identify strain-specific hemagglutinin epitopes and public stem-directed linear epitopes from influenza vaccination response data.
• Differentiate true antibody-antigen interactions from background noise using the optimized workflow's metrics.

Strengths

• Protocol validated across multiple sample types: healthy donor sera, pooled HIV-seropositive sera, and influenza-vaccinated rabbit sera.
• Enables quantitative detection of HIV epitopes across a 100-fold dilution series.
• Document provides a reproducible, generalizable workflow for enhancing viral epitope discovery and serosurveillance sensitivity.

Limitations

• Dataset is a 1.2 MB DOCX document containing methodological description, not a structured data table for direct computational analysis.
• No sample data or column definitions are provided, limiting immediate use for modeling.
• Content is specialized for PhIP-seq/VirScan methodology, requiring significant domain expertise in immunology and virology.

Provenance

Source

figshare, author Lily Kjendal.

Collection Method

Systematic evaluation of experimental variables on PhIP-seq assay performance, leading to an optimized protocol.

Time Range

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Freshness

Last updated March 25, —.

Geography

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