Supplementary Data for Linker Peptide Mechanical Clamp Study in Designed Polyprotein

A supplementary document supporting research on the 'Neighbor Peptide Effect' in a designed mechanostable protein. The file, authored by Yanwei Wang and last updated in May 2026, contains data and analysis from single-molecule force spectroscopy and steered molecular dynamics simulations. It details how a specific flexible linker peptide enhances mechanical stability in protein oligomers.

Use Cases

• Analyzing the mechanical stability enhancement (~15% increase) from oligomerization based on the described force spectroscopy results.
• Studying sequence-specific interfacial interactions, such as the hydrogen bond between Ser117 and Lys218, based on the contact-occupancy analysis.
• Investigating the role of linker peptides as external mechanical clamps in protein topology based on the described molecular mechanism.
• Validating steered molecular dynamics simulation methodologies against experimental single-molecule force data.

Strengths

• Includes specific quantitative results, such as an unfolding force increase from ~350 pN to ~400 pN.
• Provides molecular-level mechanistic insights from steered molecular dynamics simulations.
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The primary data format is a DOCX file, which may require extraction or conversion for computational analysis.

Provenance

Source

figshare

Collection Method

Data generated from single-molecule force spectroscopy and steered molecular dynamics simulations as described.

Time Range

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Freshness

Last updated 2026-05 04 05:30:24; freshness should be verified.

Geography

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