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A randomized clinical trial from 2026 by Juliano L. Gonçalves evaluated the long-term effects of systemic ceftiofur and ampicillin on resistant Gram-negative bacteria in healthy dairy cows. Fecal samples were collected from 24 cows over 12 weeks, with bacterial abundance enumerated on selective agar. The study found minimal changes in the proportion of resistant bacteria after antibiotic treatment.
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