Membrane Lipid Order and Potency Data for Therapeutic Extracellular Vesicles Under Stress

Kenichi Tamura's research data from 2026 investigates membrane lipid order as a stability-indicating metric for therapeutic extracellular vesicles (EVs). The dataset likely contains experimental results from human fibroblast-derived EVs subjected to detergent exposure, vortex mixing, freeze-thaw cycling, and lyophilization-reconstitution. It includes measurements of corrected generalized polarization (cGP), particle size, protein concentration, CD63 levels, and wound closure potency from scratch assays.

Use Cases

• Predicting potency decline in therapeutic EVs based on membrane lipid order (cGP) measurements.
• Comparing the sensitivity of lipid order metrics against conventional quality control readouts like particle size and protein concentration.
• Modeling the impact of logistics-relevant stresses (freeze-thaw, vortexing) on EV membrane integrity and function.
• Developing acceptance criteria for EV distribution control and bedside go/no-go decisions using plate reader-based assays.

Strengths

• Data is associated with a detailed methodological description covering artificial and real-world stress models.
• Released under a permissive CC-BY-4.0 license, facilitating reuse and redistribution.
• File size is 2.7 MB, indicating a manageable download and inspection process.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The primary data file is a DOCX document, which may require extraction or conversion for computational analysis.

Provenance

Source

figshare, authored by Kenichi Tamura.

Collection Method

Experimental data from human fibroblast-derived EVs isolated by ultracentrifugation and subjected to controlled stresses.

Time Range

Experimental timeframe not specified; dataset last updated in 2026.

Freshness

Last updated 2026-04-13 05:46:03; freshness should be verified.

Geography

Geographic scope of the source material is not specified.