MiR-1a-3p/Fcgr4 Axis in Osteoclast Activation and Bone Loss

Supplementary file 1 from a study on the role of the miR-1a-3p–Fcgr4 axis in pathological bone loss. The document, authored by Jiayao Zhang and last updated on 2026-05-25, contains research methods and results from experiments on RAW264.7 cells, human osteoporosis cohorts, and animal models, including a chronic unpredictable mild stress (CUMS) model.

Use Cases

• Study the regulatory relationship between miR-1a-3p and Fcgr4 based on dual-luciferase reporter assay results mentioned in the description
• Analyze osteoclast activity under different conditions based on TRAP staining, RT-qPCR, and Western blotting data described
• Investigate the impact of chronic psychological stress on bone loss based on findings from the CUMS model described
• Examine the FcγRIV–SYK–NFATc1 signaling pathway activation in osteoclasts based on immunofluorescence and Western blot results mentioned

Strengths

• File size is 10.9 MB, indicating a substantial document.
• Research includes validation across multiple models: in vitro (RAW264.7 cells), human cohorts, and animal models.
• The document is associated with a specific, detailed research hypothesis on the miR-1a-3p–Fcgr4 axis.

Limitations

• The dataset is a single 10.9 MB DOCX file; its internal structure and data formats are unknown.
• Row count and column-level documentation are absent; data semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, author Jiayao Zhang

Collection Method

Likely contains experimental data from bioinformatic prediction, cell transfection, staining, PCR, and Western blotting.

Freshness

Last updated 2026-05-25 06:03:31; freshness should be verified.