Molecular and Antimicrobial Profiles of ESBL-Producing E. coli from Egyptian UTIs

20 ESBL-producing Escherichia coli isolates from 41 clinical samples were characterized for pathotypes, virulotypes, and genotypes. Heba A. Ahmed published this supplementary document in April 2026, detailing virulence gene profiling, REP-PCR genotyping, and antibiotic susceptibility testing. The study also evaluates the antibacterial activity of biosynthesized selenium nanocomposites against these multidrug-resistant isolates.

Use Cases

• Predict antimicrobial resistance patterns from virulence gene profiles, such as the presence of the fimH gene.
• Cluster bacterial strain diversity using REP-PCR genotyping fingerprint data.
• Model the minimum inhibitory concentration (MIC) of selenium nanocomposites based on bacterial genotype or resistance phenotype.
• Analyze correlations between specific antibiotic susceptibility test results, like resistance to ampicillin and cefixime, and phylogroup classification.
• Train classifiers to identify virulent MDR ESBL-producing E. coli isolates from combined molecular characterization data.

Strengths

• Includes detailed molecular characterization data for 20 distinct ESBL-producing E. coli isolates.
• Provides concrete efficacy metrics for novel therapeutics, with minimum inhibitory concentrations of 20 μg/mL for SeNPs and 10 μg/mL for SeNCs.
• Documents multiple analysis methods: phylogrouping, virulence gene profiling, REP-PCR genotyping, and antibiotic susceptibility testing.

Limitations

• Small sample size of 20 characterized isolates from a single geographic region (Egypt), limiting statistical power and generalizability.
• Data is presented in a narrative document format (DOCX), requiring extraction for computational analysis.
• Temporal coverage of the clinical isolates is not specified, which may affect relevance to current resistance trends.

Provenance

Source

Heba A. Ahmed via figshare.

Collection Method

Clinical isolates recovered from urine and stool of hospitalized UTI patients, characterized by laboratory techniques including REP-PCR genotyping and disc diffusion tests.

Time Range

null

Freshness

Last updated in April 2026, indicating recent publication.

Geography

Isolates were collected from patients in Egypt.