Molecular Characterization of HIF-α Isoforms in Greenfin Horse-faced Filefish

Supplementary data from a study characterizing hypoxia-inducible factor alpha isoforms in the greenfin horse-faced filefish (Thamnaconus septentrionalis). The research includes phylogenetic, structural, and tissue expression analyses of three TsHIF-α genes (TsHIF-1α, TsHIF-2α, TsHIF-3α) and their response to acute hypoxia. Author Mingyu Ma published this 2.0 MB DOCX file under a CC BY 4.0 license in 2026.

Use Cases

• Analyze phylogenetic clustering and conserved bHLH-PAS domains of TsHIF-α isoforms to study evolutionary conservation.
• Compare tissue expression levels of TsHIF-1α, TsHIF 2α, and TsHIF-3α, particularly in head kidney and gill, to understand organ-specific roles.
• Model the relationship between acute hypoxia exposure and the rapid upregulation of TsHIF-3α in liver tissue relative to downstream targets vegfa and ldha.
• Examine the divergent oxygen-dependent degradation domain (ODDD) regions, including serine residue counts, for structural comparisons across species.

Strengths

• Includes precise experimental measurement of hypoxia sensitivity with a LOEcrit value of 1.43 ± 0.2 mg/L.
• Provides molecular characterization of three distinct HIF-α isoforms (TsHIF-1α, TsHIF-2α, TsHIF-3α) with phylogenetic and structural analysis.
• Data supports findings on TsHIF-3α's pivotal role in acute hypoxia adaptation, a specific regulatory mechanism.

Limitations

• Dataset is a single 2.0 MB DOCX supplementary file, not a structured, machine-readable table, limiting direct computational analysis.
• Sample data and column definitions are unavailable, requiring manual extraction of quantitative results from the document.
• Study focuses on a single fish species (Thamnaconus septentrionalis), limiting generalizability without comparative data.

Provenance

Source

figshare, authored by Mingyu Ma.

Collection Method

Experimental study involving gene cloning, phylogenetic analysis, tissue expression profiling, and dual-luciferase reporter assays.

Time Range

null

Freshness

Last updated in March 2026.

Geography

Study subject is the greenfin horse-faced filefish (Thamnaconus septentrionalis).