Supplementary File 1: Mucinase Discovery for Pseudomyxoma Peritonei Therapy

A 4.4 MB DOCX file authored by Jun Liu details the discovery of mucinases from Bacteroides thetaiotaomicron for degrading tumor-associated mucus. The document, last updated May 1, 2026, presents an integrated workflow from probiotic protease discovery to functional validation of enzymes BT4244, BT3015, and BT4272. This research provides a foundation for enzyme-targeted therapy for the rare mucinous malignancy pseudomyxoma peritonei.

Use Cases

• Identify potential enzyme targets for mucolytic cancer therapy based on the described M60 family metalloproteinases.
• Study microbial adaptation and mucus degradation mechanisms using the described cultivation of Bacteroides thetaiotaomicron with patient-derived mucus.
• Analyze transcriptomic data related to polysaccharide utilization loci (PULs) gene clusters involved in mucin breakdown.

Strengths

• File size is 4.4 MB, indicating a substantial document.
• The dataset is licensed under CC-BY-4.0, permitting broad reuse with attribution.
• The description provides specific enzyme names (BT4244, BT3015, BT4272) and a clear biological workflow.

Limitations

• The dataset is a single DOCX file; data structure and column-level information are unknown.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.
• The description is detailed but the actual data file content requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely contains research documentation, including methods, transcriptomic analysis, and functional validation results.

Freshness

Last updated 2026-05-01 05:24:46