NK Cell and Macrophage Interaction Data in Pemphigus Vulgaris

A 2.8 MB supplementary document from a study investigating the crosstalk between natural killer (NK) cells and macrophages in the autoimmune skin disorder pemphigus vulgaris. The research, authored by Vishakha Hooda and shared under a CC-BY-4.0 license, utilized flow cytometry, immunofluorescence, and in-vitro assays to examine cell profiles, activation markers, and functional changes. The file was last updated on May 8, 2026.

Use Cases

• Analyzing macrophage polarization (M1/M2) and NK cell activation states based on experimental results described.
• Studying the role of the NKG2D-MICA/B receptor interaction in immune cell crosstalk as outlined in the methods.
• Investigating changes in cytokine secretion (IFNγ, perforin) and functional markers (iNOS, CD86, CD206, arginase) from co-culture assays.
• Exploring potential therapeutic strategies for pemphigus vulgaris by targeting NK cell activity or macrophage functionality as suggested in the conclusion.

Strengths

• Provides detailed methodological description of flow cytometry, immunofluorescence, and in-vitro co-culture assays.
• Explicitly measures multiple functional outputs including cytotoxicity, phagocytic activity, and specific cytokine secretion.
• Includes results from receptor blockade experiments (NKG2D) to elucidate interaction mechanisms.
• Shared under a permissive CC-BY-4.0 license for reuse.

Limitations

• The dataset is a 2.8 MB DOCX file; the underlying numerical or image data is not directly accessible in a structured format.
• Row count and column-level documentation are absent; data semantics must be inferred from the textual description.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, author Vishakha Hooda.

Collection Method

Data generated via laboratory experiments including flow cytometry, immunofluorescence, and in-vitro cell culture assays.

Time Range

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Freshness

Last updated 2026-05-08 05:48:16; freshness should be verified.

Geography

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