Ultrasound Extraction Optimization and Cytotoxicity Data for Physochlaina Flavonoids

Zhi Ying Sun published a 574.3 KB supplementary document in 2026 detailing a botanical drug study. The research optimized ultrasound-assisted extraction of flavonoids from Physochlaina physaloides roots and evaluated their cytotoxic activity against HeLa cervical cancer cells. The document contains the full methodology and results from the 2026 study.

Use Cases

• Model flavonoid yield based on solid-liquid ratio, extraction temperature, and ultrasonic power parameters using the Box-Behnken response-surface design data.
• Analyze dose- and time-dependent cytotoxic effects on HeLa cells using MTT assay and Calcein-AM/PI staining results.
• Study apoptosis induction mechanisms by examining Annexin V-FITC/PI analysis data and western blot results for p-PI3K, p-AKT, and cleaved caspase-3 levels.

Strengths

• Document provides complete experimental methodology and results from a peer-reviewed study.
• Includes specific quantitative results, such as an optimized yield of 11.005 ± 0.663 mg/g total flavonoids.

Limitations

• Data is contained within a 574.3 KB DOCX file, not in a structured, machine-readable format like CSV.
• The dataset scope is limited to a single plant species (Physochlaina physaloides) and one cancer cell line (HeLa).

Provenance

Source

figshare, authored by Zhi Ying Sun.

Collection Method

Data generated from laboratory experiments using ultrasound-assisted extraction, Box-Behnken design, and in vitro cell assays (MTT, staining, western blot).

Time Range

Study period not specified, document published in 2026.

Freshness

Last updated in April 2026, indicating recent publication.

Geography

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