Protocol for Pancreatic Islet Calcium Imaging and Functional Beta-Cell Network Analysis

A detailed protocol for performing calcium ion imaging in isolated rodent pancreatic islets, focusing on mouse islets expressing the GCaMP6 sensor. The document provides a step-by-step guide to evaluate calcium dynamics, coordination, connectivity, and identify functional beta-cell subpopulations like first-responders, hubs, and leaders. The protocol was authored by Luis Fernando Delgadillo-Silva and last updated on April 15, 2026.

Use Cases

• Identify first-responder, hub, and leader beta-cell subpopulations based on calcium oscillation patterns described in the protocol.
• Investigate the role of calcium coordination in defective insulin secretion, a factor underlying diabetes mellitus.
• Apply the described imaging and analysis approach to other tissues where cellular coordination is critical for function.
• Study functional networks of beta-cells required for precise insulin secretion regulation.

Strengths

• The protocol is detailed and provides a step-by-step guide for a specific experimental method.
• The data is openly licensed under CC-BY-4.0.
• The file is relatively small at 878.6 KB, facilitating quick download.

Limitations

• The dataset is a protocol document (DOCX), not a primary data file; actual experimental data is not included.
• Row count and column-level documentation are unknown.
• The description is specific to the method, but the scope of any included example data is unclear.

Provenance

Source

figshare, author Luis Fernando Delgadillo-Silva.

Collection Method

Likely a documented experimental protocol for calcium imaging in pancreatic islets.

Time Range

Protocol last updated in 2026; temporal coverage of any example data is unknown.

Freshness

Last updated 2026-04-15 04:36:52.

Geography

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