Supplementary File 1: PTP-4/ATLN-1 Immune Regulation in C. elegans

A research document from figshare details a study on negative immune regulation in Caenorhabditis elegans. The 5.7 MB DOCX file, authored by Jing Xie and last updated in June 2026, describes a genome-wide RNAi screen of 166 phosphatase genes to identify the checkpoint PTP-4. It includes results from yeast two-hybrid screening, Co-IP, mutagenesis, and a small-molecule screen identifying baicalin as an inhibitor.

Use Cases

• Study negative regulatory networks in the p38 MAPK immune pathway based on the described RNAi screen.
• Investigate phosphatase-protein interactions, such as PTP-4 binding ATLN-1, using the described molecular assays.
• Identify potential drug candidates for bacterial infections based on the small-molecule screen that discovered baicalin.
• Validate structural and biochemical mechanisms of immune modulation based on the reported SPR and molecular docking analyses.

Strengths

• The study includes a genome-wide RNAi screen covering 166 phosphatase genes.
• A specific small-molecule inhibitor, baicalin, was identified and characterized with a reported binding affinity (KD = 28.69 μM).
• The document is licensed under CC-BY-4.0, permitting broad reuse.

Limitations

• The dataset is a 5.7 MB DOCX file; the underlying experimental data (e.g., raw screen results, binding data) is not directly accessible in a structured format.
• Column-level documentation is absent; field semantics must be inferred from the document text.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

figshare

Collection Method

Genome-wide RNAi screen, yeast two-hybrid screening, Co-IP, mutagenesis, and small-molecule screening followed by SPR and molecular docking.

Freshness

Last updated 2026-06-01 04:21:46; freshness should be verified.