Serratiopeptidase Production and Purification Data from Marine Bacillus Siamensis

A 305.1 KB document by Aswin Viswan, last updated April 23, 2026, details a study on producing the anti-inflammatory enzyme serratiopeptidase. The research optimized process parameters like pH, carbon, and nitrogen sources, achieving a maximum enzyme production of 3.449 U/mL at 18 hours. The enzyme was purified, showing a 3.6-fold increase in specific activity and a confirmed molecular size of 50 kDa.

Use Cases

• Optimizing fermentation conditions for enzyme yield based on tested parameters like pH, carbon source (lactose), and nitrogen source (yeast extract).
• Studying enzyme stability under different conditions based on activity data with metal ions like CaCl₂ and MgSO₄.
• Benchmarking protein purification protocols based on the described methods of ammonium sulfate precipitation, dialysis, and gel filtration chromatography.
• Validating protein identity and integrity using the reported SDS-PAGE (50 kDa) and FTIR analysis results.

Strengths

• Provides specific quantitative results, including enzyme activity levels (e.g., 3.449 U/mL at 18h) and purification fold-increase (3.6-fold).
• Describes a complete experimental workflow from bacterial growth kinetics to protein purification and characterization.
• Includes optimization data for multiple parameters: carbon source, nitrogen source, pH, inoculum size, and metal ion effects.

Limitations

• The dataset is a 305.1 KB DOCX file; the underlying structured data (e.g., raw measurements) is not directly accessible in a machine-readable format.
• Row count and column-level documentation are unknown, which may limit suitability assessment for computational analysis.
• Description metadata is limited; actual data quality and completeness require manual inspection after download.

Provenance

Source

figshare, authored by Aswin Viswan.

Collection Method

Laboratory study involving bacterial culture, enzyme activity assays, and protein purification techniques.

Freshness

Last updated 2026-04-23 05:35:06; freshness should be verified.

Geography

Marine sediment source implied, but specific location is not stated.