Supplementary file 1_Retrospective analysis of secreted PrpL protease activity in clinical

A retrospective analysis of 68 clinical Pseudomonas aeruginosa isolates, including 41 corneal and 27 non-corneal isolates, examining lysyl endopeptidase (PrpL) activity and pyocyanin production. The dataset, authored by Douglas S. Parker and last updated in May 2026, likely contains measurements from bacterial culture supernatants. The study suggests high PrpL and pyocyanin levels are more prevalent among corneal isolates and could enhance tissue damage during infection.

Use Cases

• Correlating protease activity levels with infection site based on the comparison of corneal and non-corneal isolates.
• Investigating the role of the xcp-based type II secretion system in PrpL secretion as mentioned in the results.
• Analyzing the relationship between PrpL activity and in vitro corneal epithelial cell monolayer disruption described in the study.
• Examining co-occurrence of high pyocyanin production with protease activity in clinical strains.

Strengths

• Includes data from 68 distinct clinical isolates, providing a basis for comparison.
• Focuses on a specific virulence factor (PrpL protease) with a defined experimental assay using a serine protease-specific substrate.
• Compares results from wild-type strains with secretion-defective and prpL knockout mutants for controlled analysis.

Limitations

• Row count and specific column-level documentation are unknown, which may limit suitability assessment.
• The dataset is small (335.4 KB), indicating limited scope and likely a focused experimental result set.
• Description metadata is limited; actual data quality and structure require manual inspection after download.

Provenance

Source

figshare

Collection Method

Clinical isolates were obtained from different researchers and clinicians; protease activity and pyocyanin production were measured in laboratory assays.

Freshness

Last updated 2026-05-08 05:56:29; freshness should be verified.