CRISPR Tadpole Model Data for DEE48 Epilepsy Study

347.8 KB of supplementary data from a Xenopus laevis CRISPR model investigating Developmental and Epileptic Encephalopathy 48. The dataset contains results on locomotor activity, neural calcium imaging, brain transcriptomics, and blood-brain barrier integrity from ap3b2 gene-edited tadpoles. It was authored by Suljana Banerjee and shared under a CC BY 4.0 license.

Use Cases

• Analyze locomotor activity and seizure-like episode frequency in ap3b2.S-/- tadpoles compared to sibling controls.
• Investigate interhemispheric synchrony patterns in forebrain and midbrain neural activity using GCaMP6s calcium imaging data.
• Explore transcriptomic downregulation in pathways related to ion transport, GABA neurotransmission, and blood-brain barrier function.
• Assess blood-brain barrier integrity by comparing sodium fluorescein leakage rates between CRISPant and control tadpoles.
• Evaluate the phenotypic rescue effects of acute losartan exposure on neural activity and locomotor hyperactivity.

Strengths

• Data is derived from a specific CRISPR/Cas9 gene-editing model targeting the ap3b2 ortholog in Xenopus laevis.
• Includes multi-modal experimental results: behavioral, imaging, transcriptomic, and physiological assays.
• Shared under a permissive CC BY 4.0 license, enabling broad reuse and analysis.

Limitations

• The dataset is small in scale at 347.8 KB, indicating limited scope and likely a summary of key results rather than raw experimental data.
• Specific structure, row count, and column definitions are unknown, complicating direct computational analysis.
• Data is specific to a non-mammalian model organism (Xenopus tadpoles), which may limit direct translational insights.

Provenance

Source

figshare, authored by Suljana Banerjee.

Collection Method

Generated from experiments on a Xenopus laevis CRISPR model targeting the ap3b2 gene.

Freshness

Last updated on 2026-03-23.