Serum and Salivary IgG Antibody Titers in Vaccinated Sheep

Forty-one paired serum and saliva samples from sheep vaccinated with antigens of varying complexity. The dataset compares antigen-specific IgG, IgG1, and IgG2 titers and antibody avidity between serum and saliva using ELISA and western blotting. The data was authored by Juliana Yeung and last updated on 2026-05-20.

Use Cases

• Evaluating serum as a surrogate for salivary IgG measurements based on the described correlation analysis.
• Comparing antibody responses to different antigen types (single protein, protein cocktails, methanogen mixtures) as described in the methods.
• Analyzing the relationship between serum and salivary antibody avidity for different antigens.
• Validating immunoassay methods for ruminant saliva using paired serum samples.

Strengths

• Includes 41 paired biological samples (serum and saliva), enabling direct comparison.
• Analyzes multiple antibody isotypes (IgG, IgG1, IgG2) and avidity for a detailed immunological profile.
• Uses multiple analytical techniques (ELISA and western blotting) as described in the methods.

Limitations

• Row count and column-level documentation are unknown, which limits suitability assessment.
• The description notes that methodological refinement is required before the serum-saliva avidity relationship can be confidently extrapolated across all antigen types.
• Data is contained in a 2.1 MB DOCX file, which may require extraction or conversion for analysis.

Provenance

Source

figshare

Collection Method

Paired serum and saliva samples were collected from vaccinated sheep and analyzed via ELISA and western blotting.

Freshness

Last updated 2026-05-20 05:38:33; freshness should be verified.