Spatial Transcriptomics Data on Fibroblast-T Cell Crosstalk in Allergic Rhinitis

A 2026 study by Miao Zhao provides spatial transcriptomics data from the nasal mucosa of 20 individuals. The dataset includes results from 10 allergic rhinitis patients and 10 non-allergic controls, analyzed using 10x Genomics Xenium In Situ technology. It contains findings on cell type localization, differential gene expression, and ligand-receptor interactions driving Th2 polarization.

Use Cases

• Modeling fibroblast–T cell spatial interactions based on adjacency percentages and distance thresholds (e.g., within 20 μm).
• Analyzing differential gene expression in fibroblasts based on the 187 upregulated genes identified, such as TSLP and IL33.
• Investigating correlations between molecular crosstalk scores and clinical severity metrics like serum IgE and skin prick test results.
• Validating ligand–receptor interaction axes (e.g., TSLP–IL7R, OX40L–OX40) using the provided spatial and transcriptional data.

Strengths

• Includes data from a controlled study with 10 patients and 10 controls, enabling comparative analysis.
• Integrates multimodal data, including spatial localization, gene expression, and clinical severity correlations (e.g., r=0.71 with serum IgE).
• Reports specific, validated quantitative findings, such as a 4.8x higher fibroblast–T cell crosstalk score in AR patients.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The primary data file is a DOCX document (1.4 MB), which may require extraction and conversion for computational analysis.

Provenance

Source

Miao Zhao via figshare.

Collection Method

Data generated using 10x Genomics Xenium In Situ spatial transcriptomics on human nasal mucosa samples, combined with computational analysis (CellPhoneDB/NicheNet) and qRT-PCR validation.

Time Range

Study published in 2026; specific sample collection dates are not provided.

Freshness

Last updated 2026-04-17 08:49:24; freshness should be verified.

Geography

null