Digital Light Processing Bioprinting Workflow for Methacrylated Extracellular Matrix

A supplementary document detailing a practical workflow for digital light processing (DLP) bioprinting using methacrylated porcine uterine extracellular matrix (dECM-MA). The file, authored by Hod Bruck and last updated in April 2026, describes a stepwise recipe for creating acellular constructs with 50-µm layer height and millimeter-scale geometries. It includes results from histological, biochemical, and in vitro analyses to validate the process.

Use Cases

• Replicating a DLP bioprinting protocol based on the described methacrylated dECM formulation and LAP/tartrazine recipe.
• Analyzing scaffold properties based on described metrics for dimensional fidelity, swelling behavior, and porosity.
• Benchmarking cell-matrix interaction studies based on the described in vitro tests with human uterine stromal fibroblasts.

Strengths

• The workflow is explicitly described as reproducible and practical for 405-nm DLP printing.
• Constructs are reported to have high dimensional fidelity at a 50-µm layer height and controlled swelling.
• Analysis includes specific metrics like collagen retention, sGAG retention, and quantified porosity/pore size distribution.

Limitations

• The dataset is a 22.1 KB DOCX file, indicating limited scope and likely containing textual descriptions rather than raw experimental data.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

figshare, authored by Hod Bruck.

Collection Method

Likely contains the methodology and results from the described bioprinting and analysis workflow.

Time Range

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Freshness

Last updated 2026-04-14 05:39:01; freshness should be verified.

Geography

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